3VH2
Crystal structure of Saccharomyces cerevisiae Atg7 (1-613)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-07-23 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 88.028, 88.028, 264.249 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.040 - 3.300 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vh1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.151 | 0.480 |
Number of reflections | 18578 | |
<I/σ(I)> | 27.5 | 6.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.6 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 9-13% ethylene glycol, 150mM NaCl, 20mM Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |