3VFL
Structure, Function, Stability and Knockout Phenotype of Dihydrodipicolinate Synthase from Streptococcus pneumoniae
Replaces: 3H5DExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-05-16 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.954 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 62.357, 105.332, 105.510 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.830 - 1.910 |
R-factor | 0.16779 |
Rwork | 0.165 |
R-free | 0.21241 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hij |
RMSD bond length | 0.020 |
RMSD bond angle | 2.417 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.3.0) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.550 | 74.540 | 2.030 |
High resolution limit [Å] | 1.910 | 5.710 | 1.910 |
Rmerge | 0.061 | 0.022 | 0.537 |
Number of reflections | 54089 | ||
<I/σ(I)> | 35.73 | 105.4 | 4.67 |
Completeness [%] | 99.6 | 99.3 | 97.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.2M ammonium chloride, 20% (w/v) PEG 6000, 0.1M MES pH 6.0, vapor diffusion, hanging drop, temperature 293K |