3VEK
Both Zn Fingers of GATA1 Bound to Palindromic DNA Recognition Site, P1 Crystal Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-09-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.195, 62.415, 66.046 |
| Unit cell angles | 72.39, 74.57, 73.63 |
Refinement procedure
| Resolution | 48.680 - 2.630 |
| R-factor | 0.2369 |
| Rwork | 0.235 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vd6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.921 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.680 |
| High resolution limit [Å] | 2.630 | 7.130 | 2.630 |
| Rmerge | 0.088 | 0.036 | 0.373 |
| Number of reflections | 14526 | ||
| <I/σ(I)> | 14.2 | ||
| Completeness [%] | 97.0 | 98.1 | 94.3 |
| Redundancy | 4.7 | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20% PEG 6000, 0.01M ZnCl2, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
