3VE0
Crystal structure of Sudan Ebolavirus Glycoprotein (strain Boniface) bound to 16F6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 194.859, 194.859, 194.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.930 - 3.353 |
| R-factor | 0.22523 |
| Rwork | 0.222 |
| R-free | 0.28949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3csy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.538 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.470 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.049 | 0.691 |
| Number of reflections | 17818 | |
| <I/σ(I)> | 13.4 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 300 | 15% (w/v) PEG 3350, 0.2 M lithium citrate, and 1.5% benzamidine hydrochloride, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
