3VD0
structure of p73 DNA binding domain tetramer modulates p73 transactivation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-02-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.090, 104.521, 122.994 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.950 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kmd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 43721 | |
| <I/σ(I)> | 16.5 | 4.54 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 298 | 0.1M MES, 0.1M Na acetate, pH 6.1, temperature 298.0K |
