3VAK
Structure of U2AF65 variant with BrU5 DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-01-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 166.406, 37.726, 101.631 |
| Unit cell angles | 90.00, 125.43, 90.00 |
Refinement procedure
| Resolution | 41.420 - 2.170 |
| R-factor | 0.1924 |
| Rwork | 0.188 |
| R-free | 0.22944 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.257 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
| High resolution limit [Å] | 2.160 | 4.650 | 2.160 |
| Rmerge | 0.140 | 0.094 | 0.365 |
| Number of reflections | 27427 | ||
| <I/σ(I)> | 18.7 | ||
| Completeness [%] | 98.9 | 99.4 | 92.1 |
| Redundancy | 5.7 | 5.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 1.6M Ammonium sulfate, 10% Dioxane, 0.1M MES pH 6.5, Deoxy-Big Chap, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
