3VA3
Crystal structure of RNase T in complex with a duplex DNA product (stem loop DNA with 2 nucleotide 3' overhang)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.266, 112.684, 57.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.932 - 2.714 |
| R-factor | 0.2653 |
| Rwork | 0.263 |
| R-free | 0.29040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.685 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 17286 | |
| <I/σ(I)> | 13.36 | 2.24 |
| Completeness [%] | 94.3 | 93.5 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 0.1M Sodium acetate trihydrate, 10%(w/v) Polyethylene glycol 1000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
