3VA3

Crystal structure of RNase T in complex with a duplex DNA product (stem loop DNA with 2 nucleotide 3' overhang)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribonuclease T	polymer	235	25647.0	2	UniProt (P30014) Pfam (PF00929)	Escherichia coli	Exoribonuclease T, RNase T
2	C, D (C, D)	DNA (5'-D(*GP*GP*CP*CP*CP*TP*CP*TP*TP*TP*AP*GP*GP*GP*CP*CP*TP*T)-3')	polymer	18	5474.5	2
3	E, F (A, B)	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
4	G, H, I (A, B, C)	water	water		18.0	37	Chemie (HOH)

Sequence modifications

A, B: 1 - 215 (UniProt: P30014)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 92	Glu 92	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	62243.0
Non-Polymers*	Number of molecules	2
	Total formula weight	117.9
All*	Total formula weight	62360.9

*Water molecules are not included.