3V99

S663D Stable-5-LOX in complex with Arachidonic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	51.755, 200.844, 72.184
Unit cell angles	90.00, 105.65, 90.00

Refinement procedure

Resolution	35.570 - 2.252
R-factor	0.1879
Rwork	0.186
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.661
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.570	35.570	2.380
High resolution limit [Å]	2.250	4.120	2.250
Number of reflections	62626
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	10% tacsimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K