3V4I
Crystal structure of HIV-1 reverse transcriptase (RT) with DNA and AZTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.053, 132.647, 138.014 |
| Unit cell angles | 90.00, 98.12, 90.00 |
Refinement procedure
| Resolution | 45.543 - 2.798 |
| R-factor | 0.2296 |
| Rwork | 0.229 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jsm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.380 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.798 | 2.798 |
| Rmerge | 0.083 | 0.523 |
| Number of reflections | 73202 | |
| <I/σ(I)> | 11.6 | 1.9 |
| Completeness [%] | 92.8 | 85.1 |
| Redundancy | 3.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.8 | 277 | PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE, pH 7.2, EVAPORATION, temperature 277K |
