3V20
Crystal structure of Type IIF restriction endonuclease Bse634I with cognate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGT |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-25 |
Detector | MAR555 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.937, 87.387, 125.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.556 - 2.350 |
R-factor | 0.2457 |
Rwork | 0.240 |
R-free | 0.29343 |
Starting model (for MR) | 1knv |
RMSD bond length | 0.009 |
RMSD bond angle | 1.186 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.5) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.556 | 2.460 |
High resolution limit [Å] | 2.336 | 2.340 |
Rmerge | 0.054 | 0.332 |
Number of reflections | 30943 | |
<I/σ(I)> | 22.8 | 2.3 |
Completeness [%] | 90.6 | 64.5 |
Redundancy | 4.6 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 293 | 100mM Na-acetate pH 4.25-5.5, 10mM CaCl2 or CaAc2, 4-8% (w/v) of PEG8000, VAPOR DIFFUSION, temperature 293K |