3UVR
Human p38 MAP Kinase in Complex with KM064
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2011-02-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.858, 68.246, 73.698 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.830 - 2.100 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.24480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qud |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.452 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.830 | 47.830 | 2.200 |
| High resolution limit [Å] | 2.100 | 16.000 | 2.100 |
| Rmerge | 0.091 | 0.023 | 0.386 |
| Rmeas | 0.026 | 0.430 | |
| Number of reflections | 19044 | 56 | 2446 |
| <I/σ(I)> | 17.65 | 44.51 | 5.21 |
| Completeness [%] | 99.8 | 96.6 | 99.8 |
| Redundancy | 5.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 22% PEG 4000, 50mM n-BOG, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
