3UVM
Crystal structure of WDR5 in complex with the WDR5-interacting motif of MLL4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.12718 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 78.527, 98.774, 79.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.990 - 1.570 |
R-factor | 0.1712 |
Rwork | 0.170 |
R-free | 0.19820 |
Structure solution method | SAD |
Starting model (for MR) | 2h13 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.570 | 1.570 |
Number of reflections | 42549 | |
<I/σ(I)> | 21.1 | 6 |
Completeness [%] | 98.6 | 97.8 |
Redundancy | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M ammonium sulfate, 25% PEG3350, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |