3UVK
Crystal structure of WDR5 in complex with the WDR5-interacting motif of MLL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.827, 68.496, 87.815 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.907 - 1.400 |
| R-factor | 0.15339 |
| Rwork | 0.151 |
| R-free | 0.19242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h13 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.206 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.907 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 57766 | |
| <I/σ(I)> | 47.4 | 2.7 |
| Completeness [%] | 98.3 | 81.9 |
| Redundancy | 6.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 323 | 0.1 M ammonium sulfate, 24% PEG3350, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 323K |
