3UV5

Crystal Structure of the tandem bromodomains of human Transcription initiation factor TFIID subunit 1 (TAF1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	C 2 2 21
Unit cell lengths	57.760, 199.990, 88.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.480 - 2.030
R-factor	0.192
Rwork	0.190
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ensemble of PDB entries 3HMH 2grc 2oo1 2oss 2ouo 3dai 3d7c 3dwy 3g0l 3gg3
RMSD bond length	0.015
RMSD bond angle	1.649
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.482	34.482	2.140
High resolution limit [Å]	2.030	6.420	2.030
Rmerge	0.092	0.039	0.741
Total number of observations		7243	20411
Number of reflections	33176
<I/σ(I)>	11.5	15.1	1
Completeness [%]	99.2	97.4	95.1
Redundancy	6.5	6.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1M Tris-HCl, 1.1M (NH4)2SO4, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K