3UTN

Crystal structure of Tum1 protein from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-08
Detector	RAYONIX MX-225
Wavelength(s)	0.93219
Spacegroup name	I 41
Unit cell lengths	120.939, 120.939, 48.354
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.050 - 1.900
R-factor	0.20476
Rwork	0.204
R-free	0.22755
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3olh
RMSD bond length	0.009
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.079	0.043	0.292
Number of reflections	27736
<I/σ(I)>	11.2
Completeness [%]	99.7	99.6	97.4
Redundancy	12.8	13.8	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium cacodylate trihydrate pH6.5, 0.2M, ammonium sulfate, 15% w/v PEG 8000, vapor diffusion, hanging drop, temperature 293.0K