3UP1

Crystal structure of the unliganded human interleukin-7 receptor extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-30
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	I 2 2 2
Unit cell lengths	81.424, 101.259, 151.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.930 - 2.150
R-factor	0.217
Rwork	0.214
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.150
Number of reflections	34331
Completeness [%]	99.4
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	292	1.26M (NH4)2SO4, 0.1 M Tris-HCl pH 8.5, 0.2 M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 292K