3UNW
Crystal Structure of Human GAC in Complex with Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.660, 139.614, 178.385 |
| Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
| Resolution | 29.040 - 2.560 |
| R-factor | 0.22776 |
| Rwork | 0.226 |
| R-free | 0.26137 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.923 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.040 |
| High resolution limit [Å] | 2.560 |
| Number of reflections | 75628 |
| Completeness [%] | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.3 M magnesium chloride, 0.1 M Tris, and 12% (w/v) PEG 179 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K, pH 8.5 |
