3UNF
Mouse 20S immunoproteasome in complex with PR-957
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 117.300, 194.600, 157.700 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.23724 |
| Rwork | 0.235 |
| R-free | 0.27498 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iru |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.926 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.116 | 0.547 |
| Number of reflections | 145087 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 97.1 | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2 M NaI, 40% MPD, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
