3ULV
Structure of quaternary complex of human TLR3ecd with three Fabs (Form2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-19 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 363.300, 131.660, 154.200 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 48.448 - 3.522 |
| R-factor | 0.2318 |
| Rwork | 0.231 |
| R-free | 0.25320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2A0Z 1ziw 3uls 3na9 3qpq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.613 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_896)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.208 | 0.672 |
| Number of reflections | 48292 | |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 52.8 | 11.7 |
| Redundancy | 5.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 0.1 M sodium acetate, pH 4.5, 2.54 M ammonium sulfate, 5% PEG400, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
