3UIW
Zebrafish Grx2 (APO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-12 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 92.552, 92.552, 167.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.665 - 2.601 |
| R-factor | 0.1952 |
| Rwork | 0.189 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fls |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.242 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.073 | 0.038 | 0.555 |
| Number of reflections | 8711 | ||
| <I/σ(I)> | 14 | ||
| Completeness [%] | 99.7 | 98 | 100 |
| Redundancy | 5.4 | 5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.3 M potassium sodium tartrate, 2 M ammonium sulfate, 0.1 m citrate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
