3UF2
Crystal structure of the human Colony-Stimulating Factor 1 (hCSF-1) cytokine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.150, 104.490, 116.240 |
| Unit cell angles | 90.00, 105.29, 90.00 |
Refinement procedure
| Resolution | 24.942 - 2.750 |
| R-factor | 0.1423 |
| Rwork | 0.137 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.487 |
| Refinement software | PHENIX ((phenix.refine: dev_874)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.000 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 36596 | |
| Completeness [%] | 99.5 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M magnesium chloride, 0.1M sodium citrate pH 5.0, 15% w/v PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
