3U93
Crystal structure of the GluK3 ligand binding domain complex with glutamate and zinc: P2221 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 130.401, 55.457, 88.861 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.046 - 1.881 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.24830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s50 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.918 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.910 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.049 | 0.473 |
| Number of reflections | 53068 | |
| <I/σ(I)> | 23.8 | 2.8 |
| Completeness [%] | 98.4 | 95.2 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 14% PEG 3350, 0.1 M BisTrisPropane, 0.2 M Na2SO4, 5 mM glutamate, 5 mM ZnAcetate, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
