3U8H

Functionally selective inhibition of Group IIA phospholipase A2 reveals a role for vimentin in regulating arachidonic acid metabolism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 1-BM-C
Synchrotron site	APS
Beamline	1-BM-C
Temperature [K]	77
Detector technology	CCD
Collection date	2006-10-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90020
Spacegroup name	P 31
Unit cell lengths	74.792, 74.792, 49.926
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.480 - 2.300
R-factor	0.1937
Rwork	0.192
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.103
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.932
High resolution limit [Å]	2.300
Number of reflections	13593
Completeness [%]	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.1 M Tris HCl pH 7.4, 10 mM CaCl2, 0.5 mM Beta-octyl glucoside and 4 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K