3U7D
Crystal structure of the KRIT1/CCM1 FERM domain in complex with the heart of glass (HEG1) cytoplasmic tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-18 |
Detector | Mar/Rayonix 3x3 Mosaic 225 |
Wavelength(s) | 0.873 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.050, 76.820, 79.180 |
Unit cell angles | 90.00, 113.62, 90.00 |
Refinement procedure
Resolution | 72.550 - 2.490 |
R-factor | 0.23745 |
Rwork | 0.234 |
R-free | 0.30878 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.746 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.550 | 2.620 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmerge | 0.058 | 0.312 |
Number of reflections | 26420 | |
<I/σ(I)> | 5.3 | 2.3 |
Completeness [%] | 98.7 | 97.9 |
Redundancy | 2.2 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 10% PEG 4000 and 100mM Citrate, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |