3U5O
Crystal structure of the complex of TRIM33 PHD-Bromo and H3(1-22)K9me3K14acK18ac histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 197 |
| Detector technology | CCD |
| Collection date | 2011-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.639, 63.650, 125.195 |
| Unit cell angles | 90.05, 89.98, 89.93 |
Refinement procedure
| Resolution | 28.470 - 2.700 |
| R-factor | 0.20748 |
| Rwork | 0.205 |
| R-free | 0.27972 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o33 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.729 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 52739 | |
| <I/σ(I)> | 39.6 | 2.6 |
| Completeness [%] | 98.5 | 98.4 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M sodium citrate and 20% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
