3U2Z
Activator-Bound Structure of Human Pyruvate Kinase M2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98322 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.762, 151.163, 93.213 |
| Unit cell angles | 90.00, 102.94, 90.00 |
Refinement procedure
| Resolution | 44.446 - 2.100 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.23090 |
| Structure solution method | MIR |
| Starting model (for MR) | 3gqy |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.398 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.086 | 0.040 | 0.533 |
| Number of reflections | 125358 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 98.4 | 96.3 | 91.8 |
| Redundancy | 3.5 | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 291 | 25% PEG-3350, 0.2M ammonium sulfate, 0.1M Bis-Tris. Activator concentration: 0.01M., pH 6.5, vapor diffusion, temperature 291K |
