3U2Q

EF-Tu (Escherichia coli) in complex with NVP-LFF571

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-03-21
Detector	ADSC QUANTUM 210
Spacegroup name	P 21 21 2
Unit cell lengths	83.303, 132.473, 37.433
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.020 - 2.700
Rwork	0.233
R-free	0.31500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d8t
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.099	0.416
Number of reflections	11365
<I/σ(I)>	9.7	2.79
Completeness [%]	93.5	77.2
Redundancy	6.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.23	293	50mM Tris PH8, 50mM NaCl , pH 8.23, VAPOR DIFFUSION, SITTING DROP, temperature 293K