3U10

Tetramerization dynamics of the C-terminus underlies isoform-specific cAMP-gating in HCN channels

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8726
Spacegroup name	P 4 21 2
Unit cell lengths	96.730, 96.730, 50.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.930 - 2.300
R-factor	0.20377
Rwork	0.200
R-free	0.27150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q43
RMSD bond length	0.021
RMSD bond angle	1.899
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.030	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.128
Number of reflections	11143
Completeness [%]	99.8	100
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	278	20% PEG 8000, 0.5M NaCl, 0.1M Citrate buffer (pH 4.6), 10% glycerol, VAPOR DIFFUSION, temperature 278K