3TXO
PKC eta kinase in complex with a naphthyridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99180 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.533, 56.861, 63.367 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.890 - 2.050 |
| R-factor | 0.19582 |
| Rwork | 0.194 |
| R-free | 0.24100 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.982 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.370 | 2.103 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.091 | |
| Number of reflections | 25157 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | FREE INTERFACE DIFUSION | 7.8 | 298 | 1 uL protein (18mg/ml in 0.25 M NaCl, 0.025 M Tris pH 7.8, 4mM TCEP, 1mM EDTA) with 3-4 uL crystallisation buffer (25 % PEG3350 w/v, 200 mM citrate), FREE INTERFACE DIFUSION, temperature 298K |
