3TWK

Crystal structure of arabidopsis thaliana FPG

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97930
Spacegroup name	P 41 21 2
Unit cell lengths	93.054, 93.054, 157.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.222
R-free	0.27320
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.303
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Rmerge	0.090
Number of reflections	31529
<I/σ(I)>	22.6
Redundancy	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	285	24% (w/v) PEG 3350, 175 mM MgCl2, and 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	8	285	24% (w/v) PEG 3350, 175 mM MgCl2, and 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	8	285	24% (w/v) PEG 3350, 175 mM MgCl2, and 100 mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K