3TW4

Crystal Structure of Human Septin 7 GTPase Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-09-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97627
Spacegroup name	P 61
Unit cell lengths	82.510, 82.510, 210.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.420 - 3.350
R-factor	0.2927
Rwork	0.289
R-free	0.36520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qnr
RMSD bond length	0.008
RMSD bond angle	1.544
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.420		3.550
High resolution limit [Å]	3.350	9.800	3.350
Rmerge	0.049	0.012	0.660
Number of reflections	11558	470	1825
<I/σ(I)>	15.1	66.17	1.59
Completeness [%]	98.7	97.7	97.8
Redundancy	3.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.4	291	100mM Tris pH 8.4; 16% Glycerol; 1.5M Amonium Sulphate; 500mM GDP; 12.5% Isopropanol and 7mg/mL SETPT7G, VAPOR DIFFUSION, HANGING DROP, temperature 291K