3TVK
Diguanylate cyclase domain of DgcZ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-21 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 62 |
Unit cell lengths | 79.623, 79.623, 51.207 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
R-factor | 0.167 |
Rwork | 0.166 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w25 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.281 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.210 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.075 | 0.346 |
Number of reflections | 17292 | |
<I/σ(I)> | 24.1 | 7.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.5 | 12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 293 | 100mM Tris, 22.5% PEG 4000, pH 8.5, vapor diffusion, hanging drop, temperature 293K |