3TV1
Crystal structure of RtcA.AMP product complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2011-04-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.282 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.160, 79.810, 85.670 |
Unit cell angles | 90.00, 103.65, 90.00 |
Refinement procedure
Resolution | 36.907 - 1.900 |
R-factor | 0.1827 |
Rwork | 0.180 |
R-free | 0.23260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kgd |
RMSD bond length | 0.007 |
RMSD bond angle | 1.103 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.910 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 52851 | |
Completeness [%] | 98.2 | 91.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 28% PEG5000 MME, 150 mM Ammonium sulfate, 100 mM MES pH6.5, 10 mM AMP, 15 mM sodium pyrophosphate, 30 mM Manganese chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |