3TQ6

Crystal structure of human mitochondrial transcription factor A, TFAM or mtTFA, bound to the light strand promoter LSP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 21 21 2
Unit cell lengths	113.900, 117.200, 56.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.840 - 2.450
R-factor	0.1832
Rwork	0.182
R-free	0.22830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.840	40.000	2.570
High resolution limit [Å]	2.450	7.750	2.450
Rmerge	0.080	0.031	0.467
Number of reflections	28529
<I/σ(I)>	8.2	40.7	4.2
Completeness [%]	99.8	98.3	100
Redundancy	7	6.1	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293.15	37.5% (v/v) PEG1000, 300 mM sodium chloride, 0.1 M Na,K-phosphate, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K