3TPO
Crystal structure of D192A/E396A mutant of mouse importin alpha2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-27 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.343, 89.763, 99.824 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.940 - 2.100 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.19170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ial |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.419 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.930 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 41730 | |
| Completeness [%] | 99.8 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.1M HEPES, 1.0M KNa-tartrate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
