3TPM
Crystal structure of MAL RPEL domain in complex with importin-alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-27 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.658, 90.116, 98.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.000 - 2.100 |
| R-factor | 0.1681 |
| Rwork | 0.166 |
| R-free | 0.20800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ial |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.340 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 41177 | |
| Completeness [%] | 99.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 293 | 50mM Tris-HCl, 1.4M Li2SO4, pH 7.4, VAPOR DIFFUSION, temperature 293K |
