3TOP

Crystral Structure of the C-terminal Subunit of Human Maltase-Glucoamylase in Complex with Acarbose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	105.501, 105.501, 516.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.447 - 2.881
R-factor	0.2219
Rwork	0.218
R-free	0.28410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qly
RMSD bond length	0.009
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.980
High resolution limit [Å]	2.880	2.880
Number of reflections	59475
Completeness [%]	87.9	87.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.3 M MgSO4,16% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K