3TLY
Microcin C7 self immunity protein MccF active site mutant S118A/N220A/K247A in the apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.422, 85.658, 73.016 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tla |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.720 |
| High resolution limit [Å] | 1.690 | 4.590 | 1.690 |
| Rmerge | 0.063 | 0.048 | 0.210 |
| Number of reflections | 73757 | ||
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 100.0 | 99.4 | 99.9 |
| Redundancy | 6.1 | 6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 10% PEG 8000, 8% ethylene glycol, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
