3TB8

Crystal structure of full-length myristoylated HIV-1 Nef

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-07-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	89.330, 89.330, 27.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.250 - 3.710
R-factor	0.2334
Rwork	0.231
R-free	0.33660
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (- Phaser)
Refinement software	PHENIX (1.6.2_432 - Refine)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.250
High resolution limit [Å]	3.710
Number of reflections	2622
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	288	25% polyethyleneglycol 3350, lithium sulfate, HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K