3T8V

A bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-07
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	75.542, 108.821, 118.274
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.040 - 1.800
R-factor	0.1622
Rwork	0.160
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.570
High resolution limit [Å]	1.800
Number of reflections	90083
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	22%(v/v) PEG8000, 10%(v/v) Glycerol, 0.1M Tris, 0.2M MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K