3T6A

Structure of the C-terminal domain of BCAR3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2011-01-28
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.233, 151.889, 196.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.761 - 2.400
R-factor	0.1772
Rwork	0.174
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.053
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.750
High resolution limit [Å]	2.400
Rmerge	0.096
Number of reflections	59059
Completeness [%]	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	298	15-20 % PEG3350, 0.04 M citric acid and 0.06 M Bis-Tris propane (pH 6.4), VAPOR DIFFUSION, SITTING DROP, temperature 298K