3T36

Crystal structure of lytic transglycosylase MltE from Eschericha coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8350
Spacegroup name	P 21 21 21
Unit cell lengths	77.764, 94.925, 160.494
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.770 - 2.250
R-factor	0.1923
Rwork	0.189
R-free	0.22350
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.174
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.770
High resolution limit [Å]	2.250
Number of reflections	56663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M lithium sulfate, 15% PEG8000, 20 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K