3T2N
Human hepsin protease in complex with the Fab fragment of an inhibitory antibody
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-09-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9188 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.980, 66.580, 108.330 |
| Unit cell angles | 88.71, 94.30, 104.53 |
Refinement procedure
| Resolution | 47.330 - 2.550 |
| R-factor | 0.2442 |
| Rwork | 0.242 |
| R-free | 0.27520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 54655 | |
| <I/σ(I)> | 7.96 | 2.13 |
| Redundancy | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 18% PEG 3350, 0.15 M MgSO4 and 0.01 M barium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
