3T1N
Structure of human MICROCEPHALIN (MCPH1) TANDEM BRCT domains in complex with a CDC27 phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-09-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 38.201, 46.506, 55.322 |
Unit cell angles | 86.06, 70.69, 89.67 |
Refinement procedure
Resolution | 28.710 - 2.600 |
R-factor | 0.2322 |
Rwork | 0.231 |
R-free | 0.26230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1t29 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.669 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.710 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.100 | 0.340 |
Number of reflections | 10161 | |
<I/σ(I)> | 5.6 | 2.2 |
Completeness [%] | 92.2 | 94.9 |
Redundancy | 2.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 30% PEG 3350, 0.3 M NACL, 0.1 M BIS-TRIS, PH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 295K |