3T0O
Crystal Structure Analysis of Human RNase T2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184,0.9810 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.200, 67.400, 47.900 |
Unit cell angles | 90.00, 90.81, 90.00 |
Refinement procedure
Resolution | 27.560 - 1.590 |
R-factor | 0.15378 |
Rwork | 0.152 |
R-free | 0.19033 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dix |
RMSD bond length | 0.021 |
RMSD bond angle | 2.057 |
Data reduction software | XDS |
Data scaling software | SADABS |
Phasing software | PHASER (/ SHELXE) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.310 | 1.690 |
High resolution limit [Å] | 1.585 | 1.590 |
Rmerge | 0.063 | 0.191 |
Number of reflections | 26433 | |
<I/σ(I)> | 171 | 5.04 |
Completeness [%] | 98.0 | |
Redundancy | 5.91 | 2.55 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 15% PEG3350, 0.2 M ammonium nitrate, macroseeding, no buffer, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |