3SZQ
Structure of an S. pombe APTX/DNA/AMP/Zn complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2010-07-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.2830 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 157.050, 157.050, 157.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.832 - 2.353 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.21100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.062 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.663 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.102 | 0.462 |
| Number of reflections | 14067 | |
| <I/σ(I)> | 20.9 | 4.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 297 | 0.2 M sodium/potassium tartrate, 20% w/v PEG3350, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
