3SYA

Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with sodium and PIP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-21
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 4 21 2
Unit cell lengths	85.851, 85.851, 177.862
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.727 - 2.980
R-factor	0.2419
Rwork	0.240
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e4f
RMSD bond length	0.010
RMSD bond angle	1.242
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.727	41.727	3.110
High resolution limit [Å]	2.975	6.460	3.000
Rmerge	0.108	0.048	0.827
Number of reflections	13919
<I/σ(I)>	10.2
Completeness [%]	97.4	91.4	99.1
Redundancy	5.8	5.5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	50 mM sodium citrate, pH 6.0, 1 M sodium chloride, 20% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K