3SV3
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with the artificial base pair dNaM-d5SICSTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-12-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.545, 108.545, 90.978 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.001 - 2.100 |
R-factor | 0.1698 |
Rwork | 0.167 |
R-free | 0.21540 |
Starting model (for MR) | 3m8s |
RMSD bond length | 0.008 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.7.1_743) |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.230 |
High resolution limit [Å] | 2.100 | 2.100 |
<I/σ(I)> | 12.41 | 2.33 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 9.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 13% PEG 8000, 100mM NH4Cl, 0.1M MgFormate, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |