3STA
Crystal structure of ClpP in tetradecameric form from Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.572, 96.292, 192.614 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.280 |
| R-factor | 0.22641 |
| Rwork | 0.224 |
| R-free | 0.26946 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.993 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 155453 | |
| Completeness [%] | 95.3 | 76.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 10% PEG 3000, 100mM cacodylate, 200mM magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
